| Study on the triglycerides of cotton bollworm, Helicoverpa armigera Hub. (Lepidoptera: Noctuidae) fed on the four different diets |
| Paper ID : 1321-3IICE (R1) |
| Authors: |
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homa alad *1, reza farshbaf pour abad2, Masoud Darabi3 1agriculture, plant protection, univercity of tabriz, tabriz, iran 2Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran 3Department of Biochemistry and Clinical Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran |
| Abstract: |
| Helicoverpa armigera Hub., commonly known as cotton bollworm is a major polyphagous noctuid pest in Asia. The insect fat body plays an essential role in energy storage and utilization. Lipid is the main fat body component, and more than 90% of the lipid is stored as triglyceride. Fatty acids stored as triglyceride can be used for energy production through β-oxidation. The amount of fatty acid incorporated by the fat body is dependent on the developmental stage and feeding status of the insect. In the present study, we evaluated the fatty acid composition of triglycerides forming the total body fat of the 6th instar larvae of cotton bollworm reared on four different diets. A thin layer chromatography (TLC) technique and a liquid-gas chromatography (GLC) were used for extraction and isolation of fatty acids of phospholipids. By using these methods, a mixture of fatty acids of different lengths and number of double bonds can be completely separated. Results showed that in the triglyceride group the highest fatty acid were monounsaturated fatty acids (MUFAs) and saturated fatty acids (SFAs) in the amount of 45.76 and 44.2%, respectively. The highest amount of triglyceride was associated in MUFAs with oleic acid (18:1) on tomato, potato, and green bean which were detected 41.73, 40.76, and 40.4 %, respectively. In SFAs the highest fatty acid was palmitic acid (16:0) with amount of 36.36% on artificial diet. The fatty acids of eicosadienoic acid (20:2) and arachidic acid (20:0) were reported as the lowest on all four diets with a value of 0.1, and 0.35%, respectively. Consequently, the fatty acids composition was changed with respect to the cotton bollworm diet. |
| Keywords: |
| Keywords: Cotton bollworm, Fatty acid, Triglyceride, TLC, GLC |
| Status : Paper Accepted (Poster Presentation) |
