| Investigation of resistance to abamectin in Iranian populations of western flower thrips, Frankliniella occidentalis |
| Paper ID : 1206-3IICE |
| Authors: |
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Zahra Gholami *1, Khalil Talebi Jahromi1, Vahid Hosseininaveh1, Hadi Mosallanejad2 1Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. 2Pesticide Research Dept., IRIPP |
| Abstract: |
| In current study resistance to abamectin was detected and confirmed in greenhouse populations of western flower thrips, Frankliniella occidentalis from eight areas of Iran including Tehran, Markazi, Alborz, Qazvin, Isfahan, Yazd M, Yazd B and Kerman. As a first step to understand the level of resistance, LC50 value of abamectin on 2nd instar larvae was estimated by green bean pod sections-dipping method. At least five concentrations and three replicates were used per concentration. Mortality was recorded 48 hours after treatment. Results of dose-mortality bioassays revealed resistance ratios ranging from 13.19 to 59.57-fold for abamectin. Elevated LC50 value was obtained from Alborz strain with the highest resistance ratio when compared to the susceptible population, Isfahan. Synergism experiments with piperonyl butoxide (PBO) (P450 inhibitor) and S,S,S-tributyl-phosphorotrithioate (DEF) (esterase inhibitor) in a concentration of 1000 mg/l performed to demonstrate the role of metabolic resistance. Based on the the results, PBO reduced the resistance ratios for seven populations although there was a higher reduction in resistance of Alborz population exposed to TPP (4.88 fold). These results suggest the role of the detoxifying enzyme, multifunction oxidases (MFO) might be involved as a resistance mechanism. We suggest that abamectin should be used with cautiousness in the management program of western flower thrips. |
| Keywords: |
| Frankliniella occidentalis, abamectin, Alborz, Isfahan, resistance |
| Status : Paper Accepted (Poster Presentation) |
