| Metabolic resistance related to detoxifying enzyme activity in Iranian populations of Frankliniella occidentalis |
| Paper ID : 1205-3IICE |
| Authors: |
|
Zahra Gholami *1, Khalil Talebi Jahromi1, Vahid Hosseininaveh1, Hadi Mosallanejad2 1Department of Plant Protection, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. 2Pesticide Research Dept., IRIPP |
| Abstract: |
| The western flower thrips, Frankliniella occidentalis (Pergande), is a serious pest of greenhouse crops in Iran owing to direct and indirect damages due to feeding on the host plants and transmission of important plant viruses and its very high potential of resistance to insecticides. In this study, metabolic resistance to dichlorvos and abamectin were examined in greenhouse populations of F. occidentalis collected from Tehran, Markazi, Alborz, Qazvin, Isfahan, Yazd (M and B) and Kerman with different resistance levels. Accordingly, esterase activity towards alpha-naphthyl acetate and beta-naphthyl acetate, glutathione S transferase (GST) activity towards 1-Chloro-2, 4-dinitrobenzene, cytochrome P450 monooxygenase activity towards 7-ethoxycoumarin (ECOD) and p-nitroanisole (PNOD) and normal acetylcholine esterase activity by acetylcholine iodide were assayed. Also, a non-denaturing gel electrophoresis of esterase activity was performed. Esterase activity in Yazd populations (resistance populations to dichlorvos – RF = 2.14 and 2.04 fold) was significantly higher than in the reference population (Isfahan) for both model substrates (Activity Ratio = 1.69 and 7.31 fold). ECOD activity in resistance population to abamectin (Alborz – RF = 59.57 fold) was 1.2 fold higher than Isfahan population whereas PNOD activity in Markazi population (RF = 16.71 fold) was higher than Alborz population. The highest GST activity was observed in Kerman population (AR = 1.33 fold) and normal acetylcholine esterase activity in Yazd B population (AR = 14.16 fold) was higher than others. Analysis of esterase zymogram showed that the highest intensities of esterase bands were for Yazd M and Yazd B. the results confirmed that cytochrome P450 is interfered in abamectin resistance and also resistance to dichlorvos is due to increased activity of esterase and GST activity in resistance populations. Therefore it can be suggested that the use of these two insecticides will be considered with carefulness. |
| Keywords: |
| abamectin, metabolic, resistance, activity, esterase, cytochrome P450 monooxygenase |
| Status : Paper Accepted (Poster Presentation) |
