| First report of Steinernema carpocapsae from Moghan region and its biocontrol potential against Agrotis segetum in laboratory condition |
| Paper ID : 1182-3IICE (R1) |
| Authors: |
|
Laleh Ebrahimi *1, Zahra Tanhamaafi2, Parviz Sharifi Ziveh3 1BioControl Research Dept., Iranian Research Institute of Plant Protection, Tehran, Iran 2Nematology Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran 33Plant Protection Research Department, Ardabil Agricultural and Natural Resources Research and Education Center, AREEO, Moghan, Iran, |
| Abstract: |
| During a survey of entomopathogenic nematodes (EPNs) in Moghan region of Iran in 2015, a steinernematid species was isolated using the Galleria-baiting method. Based on its morphological and phylogenetic analysis of molecular data, the isolate was identified as Steinernema carpocapsae. The ITS rDNA sequence was deposited in NCBI with accession number of MF187617. Nucleotide row data was edited using MEGA 6.0 software and Homologous sequences were involved in analysis using Blast software. Sequences were aligned using Clustal W. Bootstrap analysis was conducted. The phylogenetic tree was constructed by the maximum likelihood method using MEGA 6.0 software and Steinernema feltiae was used as outgroup. The common cutworm, Agrotis segetum is one of the most important and destructive pests in Moghan, therefore the lethal effect of S. carpocapsae isolate Moghan was evaluated in a soil assay against last instar larvae of A. segetum in the laboratory conditions. Rearing and bioassay conditions were 26±1° C and relative humidity of 70 ± 10% and photoperiod 16: 8 (L: D). Plastic cups (9 cm height and 7.5 cm diameter) which were filled with 250 g autoclaved moist sandy soil (85% sand, 10% silt and 5% clay and 10% moisture (w/w) were used as experimental units. Infective juveniles were added in certain concentrations in 0.5 ml of water to the surface of the soil, separately. Finally, the individual larva was placed on the soil and the cups were covered with ventilated lids to avoid desiccation. Control cups received 0.5 ml of distilled water. Fifteen cups were used for each nematode concentration and the control. The experiment was replicated three times. The cadavers were counted after 7 days. The bioassay results showed high susceptibility of the larvae to S. carpocapsae. The LC10, LC50 and LC90 values were 9.9, 54.13, and 246.2 IJs (infective juveniles) per larva of the pest, respectively (Χ2= 7.36; df=3, P value= 0.061). |
| Keywords: |
| ITS-rDNA, biological control, common cutworm, LC50, Entomopathogenic nematode |
| Status : Paper Accepted (Poster Presentation) |
