| Investigation on gut symbiotic bacteria of Mediterranean flour moth Anagasta kuehniella |
| Paper ID : 1179-3IICE (R3) |
| Authors: |
|
Maryam Jahanbashi *1, Solmaz azimi1, Naser Eivazian Kary2, Vahid Fallahzadeh Mamaghani1 1Department of Plant Protection, College of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran 2Dep. of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran |
| Abstract: |
| Many insects harbor large communities of diverse microorganisms which play important roles for insect nutrition, reproduction, development, behavior and resistance to pathogen colonization. Symbiotic bacteria can also indirectly affect viral transmission by enhancing immunity and resistance to viruses in insects. Studying the insects’ microbiota can help us better understand their ecology and offer insights into developing new pest control strategies. Here, the microbiome of last instar larvae of Anagasta kuhniella Zeller (Lepidoptera: Pyralidae) was evaluated in term of taxonomic diversity. First, larval body surface was sterilized using ethanol (75%) and their whole gut was removed and homogenized. The homogenized tissue was cultured on Luria Bertani Agar (LBA) to obtain facultative symbiotic bacteria. The cultures were incubated at 28 ºC for 48-72 h. After sub-culturing the single colonies, two replications of each sample were subjected to molecular study. Phylogenetic analysis was conducted based on 16s-rRNA sequence of studied isolates for species identification. After amplification of mentioned region by universal primers, PCR products of five samples were subjected to direct sequencing. The chromatogram files of resulting sequences were edited by Chromas lite, aligned using Clustal X and consensus sequence was subjected to blast search to find homologous sequences in GeneBank. The resulting data matrix from aligned homologous sequences by Clustal X was subjected to phylogenetic reconstruction using maximum parsimony, maximum likelihood and neighbor joining methods by MEGA 6 software. Based on monophyletic groups of the reconstructed phylogenetic tree using MP, ML and NJ methods the strains were identified as Bacillus sp. and Enterococcus munditii. Resulted 16s-rRNA sequences of bacteria were deposited in NCBI with accession numbers MN068418 and MN068419. |
| Keywords: |
| Symbiont bactertia, Anagasta kuehniella, Larvae, Lepidoptera |
| Status : Paper Accepted (Poster Presentation) |
