Cold storage of green lacewing, Chrysoperla carnea (Neuroptera: Chrysopidae) eggs: effects on survival and development parameters
Paper ID : 1175-3IICE
Authors:
fatemeh honarmandgolboteh *
iran Mashhad
Abstract:
Common green lacewing, Chrysoperla carnea (Stephens) is a polyphagous predator of many different agricultural pests and has a great potential for mass rearing and augmentative releasing in greenhouse and open fields. In this study, the storage feasibility of this predator’s eggs at low temperature and the effects of cold storage on egg hatching and survival and development of the emerged larvae and adults were assessed. Larvae and adults of C. carnea was reared on Anagasta kuehniella eggs and honey solution respectively, and then populations of both one-day and two-days old eggs were stored separately in a refrigerator (5±1˚C, and full darkness) for 0 (control), 3, 5, 7, 10, 14, 21 and 30 days. Egg hatching rate and survival percent of larvae decreased significantly with increasing cold storage duration in both one-day and two-day old stored eggs. But, larva and pupa developmental time were not significantly affected by low temperature and storage duration and ranged from 7.66 to 8.35 and 8.26 to 9.47 days, respectively. Egg hatching decreased significantly from 50±3.96 percent in control (unstored eggs) to 0 percent in 30-days storage duration, while the egg hatching percent in 5 and 7 days of storage (in one-day and two-day old egg, respectively) had no significant difference with control. Moreover, larval survival percent decreased from 95.6±2.5 percent in control to 52.95 and 25 percent in one-day and two-day old stored eggs, respectively. In conclusion, C. carnea eggs can be cold stored for up to 7 days without a significant adverse effect on their hatching and survival of emerged larvae. These findings could be useful for mass rearing and cold storing of this predator in commercial insectaries and having a sufficient number of eggs to release in fields.
Keywords:
Chrysoperla carnea, Cold storage, Eggs, Development, Survival
Status : Paper Accepted (Poster Presentation)