| Effects of Bacillus thuringiensis subsp. tenebrionis ,Henna, Lawsonia alba essence Neemarin (Neem, Axadirachta indica A) and Fenvalerate on adult stage of Chaetocnema tibialis under laboratory and field conditions |
| Paper ID : 1156-3IICE (R1) |
| Authors: |
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Mahsa Abdollahzadeh Bavani *1, Shahram Aramideh2, Shima Hosseinzadeh3 1Department of entomology, faculty of plant protection, urmia university of iran 2. Assistant Professor of Plant Protection Department, Agriculture Faculty, Urmia University, Iran 3MS Student of Plant Protection Department, Agriculture Faculty, Urmia University, Iran. |
| Abstract: |
| The chemical control of the pests causes environmental contamination, the death of natural enemies and serious dangers to human health, so the use of bio-pesticides is essential. In this research effect of Bacillus thuringiensis subsp. tenebrionis (Novodor®) with Henna and Neemarin in comparison with Fenevalerat on Chaetocnema tibialis in the laboratory condition (25±2 ºC, 57±2 RH and 16: 8 L: D) and field conditions were evaluated. Adults flea beetle collected from fields in three leaves stage of sugar beet by aspirator. Probit analysis was conducted to determine the lethal concentration of each compound in the laboratory conditions. Bioassay was carried out using leaf disc methods in laboratory condition. For field trail GLM-U analysis using the Henderson-Tilton formula was performed for assessing the individual and combined effects of compounds at 1, 3, 7 and 14 days after treatment. The LC50 values of Bt alone, Bt in combination with Neemarin, Bt in combination with Henna and Fenevalerat after 24, 48 and 72 hours were (2972.65, 2222.76, 3847.37 and 272.39), (1477.34, 2092.13, 2855.84 and 138.81) and (513.79, 1854.26, 2145.20 and 75.18) ppm at lab condition, respectively. In the field study, the mortality of Bt alone, Bt mixed with Henna (%) and Bt mixed with Neemarin and Fenevalerat after 7 and 14 days were (38.47±2.32, 44.37±2.32, 42.37±2.32 and 70.47±2.32), (42.06±3.01, 52.53±3.01, 50.03±3.01 and 79.78±3.01%, respectively). Also in damage assessment, the least damage were in Fenevalerat and Bt mixed with Henna and Neemarin treatments (4.66, 11.66 and 16.33%) and most damage in Henna treatment after control (28.33 and 32%). The results of this study showed that the Bt can be used in combination with Neemarin and Henna in the integrated management program of this pest in order to reduce pesticide usage. |
| Keywords: |
| Bacillus thuringiensis, Neemarin, Henna, Fenvalerate, Chaetocnema tibialis, Damage |
| Status : Paper Accepted (Poster Presentation) |
