Effects of Hexaflumuron and Lufenuron on Intermediary Metabolism of American bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
Paper ID : 1116-3IICE (R1)
Authors:
Mahdieh khorshidi *1, reza farshbaf pour abad1, moosa saber1, arash zibaee2
1Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2Department of Plant Protection, Faculty of Agriculture, University of Guilan, Guilan, Iran
Abstract:
Intermediary metabolism shows various pathways in which digested foods such as carbohydrates, lipids and proteins are processed to generate energy. It is known that some chemicals may interfere in this pathway and affect growth and development of target insects. Thereby, effects of two chitin synthesis inhibitor, hexaflumuron and lufenuron were determined on intermediary metabolism of 3rd instar larvae H. armigera via assessments of various enzymatic and non-enzymatic compounds. Effects of the insecticides were assessed by incorporating different concentrations of the given compounds into the artificial diet. Results showed that the activity of alanine (ALT) and aspartate (AST) aminotransferases in larva exposed to LC10 of insecticides significantly increased in comparison with control after 24 h. Activity of γ-glutamyl transferase (GGT) in the treated larvae was higher compared to that in control with no statistical differences. The treatment of 3rd instar larvae of H. armigera with both two insecticides caused significant increase in activity level of acid and alkaline phosphatases (ACP, ALP) and lactate dehydrogenase (LDH). In addition to enzymatic compounds, triacylglyceride, glycogen and protein, as storage macromolecules, were significantly changed in treated larvae compared to those of control. Results of current study clearly revealed the possible interference of chitin synthesis inhibitors in biochemical composition of H. armigera in comparison with control.
Keywords:
Hexaflumuron,Lufenuron, Intermediary Metabolism, Aminotransferase, Triacylglyceride
Status : Paper Accepted (Poster Presentation)