| Effect of fumigant and Digestive toxicity of essential oils from two growth stages of Artemisia annua on cellular immune system of lesser mulberry pyralid, Glyphodes pyloalis |
| Paper ID : 1080-3IICE (R1) |
| Authors: |
|
marziyeh oftadeh *1, Jalal Jalali Sendi2, asgar ebadollahi3 1Rasht, Persian Gulf Highway, 5th km of Qazvin Road, Guilan University Complex
Postcode 4199613776 2University of Guilan 3University of Mohaghegh Ardabili Natural Resources Moghan |
| Abstract: |
| In contrast to synthetic insecticides that have problem of resistance and high residual levels, the use of natural insecticides has long been thougth as an attractive alternative to synthetic chemical insecticides. Many essential oils of plant have a broad spectrum of activity against insects ranging from insecticidal, anti-feedant, repellent, oviposition deterrent, growth regulatory and their immune functions. The genus Artemisia with more than 300 different species is a member of a large plant family Asteracea (Compositae) encompassing. Extract and essential oil of Artemisia annua have shown considerable toxicity on several insect pests along with different physiological disorders on immune responses, nutrition, reproduction, and intermediary metabolism.This study was carried out to evaluate the effects of essential oils isolated from vegetative and flowering stages of A. annuaon on the cellular immunity and phenoloxidase activity of the 4th instar larvae of Glyphodes pyloalis through fumigant and digestive exposure. Total and differentiate hemocyte numbers, nodule formation and phenoloxidase activity before Beauveria bassiana and latex beads were significantly decreased in comparison with controls and a clear dose-response relationships was established with respect to immune reactions. THC reached to 312.33 and 342.66 (cells ×10 4 /ml) in lc50 after treating the larvae with digestive toxicity in Vegetative and Flowering stage, respectively. Results showed that vegetative stage stage extract was more effective compared with that of flowering stage in damaging on immune system of the insect, also in this study obviously showed that fumigation application of the oils was more effective in decreasing immunity than digestive. Therefore, it is concluded that essential oils of A. annua provided effective control of Glyphode spyloalis through negative effects on its immune cells. The affected insects may be more vulnerable to insect pathogens due to their decremented immunity. |
| Keywords: |
| synthetic insecticides, essential oils, Artemisia annua, immune system, Glyphodes pyloalis |
| Status : Paper Accepted (Poster Presentation) |
