| Identification of up-regulated proteins after feeding in the gut of new adult sunn pest using Proteomics technique |
| Paper ID : 1068-3IICE (R1) |
| Authors: |
|
Mohammad Saadati *1, Mohammad Mehdi Rabieh2 1Plant protection 2Department Of Plant Protection-Faculty of Agriculture-University of Birjand-Birjand-Iran |
| Abstract: |
| Bottom-up proteomic technique is a usual way to identify difference proteins in the insect’s world. Digestive system of the insects consists from the salivary gland and alimentary canal. Proteome patterns of some of organs in the adult sunn pest and fifth- instar nymphs were determined using two dimensional electrophoresis by authors. The study of insect digestive system particularly sunn pest has a key role for finding new potential targets in enzyme-inhibitor interaction. Any disruption in the normal process of insect nutrition can be reduced or suppressed feeding and assimilation in the insects. In this study, new adult sunn pest were collected from wheat farm around Torbat e Jam, Iran. After 2- days feeding, twenty adults (10 male and 10 female) were selected for dissection. Midgut proteins visualized by two dimensional electrophoresis patterns. There were many spots (671 spots) in the proteome patterns that need to be identified. The sixty and seven spots that changed after feeding were selected as target proteins. These spots cut and digest with trypsin and then injected to liquid chromatography and mass spectrometry (LC/ MS-MS). After analysis of resulted spectrums using National center of biotechnology information with two enteries Animals and wheat. Results showed that α-amylse, different proteases, catalase, enolase and glyceraldehis3-phosphate were up regulated in the gut of fed sunn pest in comparison to unfed adults. This result suggests that protein expression in the gut of sunn pest is affected with feeding directly. Although some of gut proteins were identified by the authors in the gut of sunn pest, but this study showed amount of changed proteins in the digestive system before and after feeding. |
| Keywords: |
| Proteome, Inhibitor, Two dimensional electrophoresis, Mass spectrometry |
| Status : Paper Accepted (Poster Presentation) |
