Nanoemulsions of eucalyptus essential oil against Tribolium castaneum
Paper ID : 1053-3IICE (R1)
Authors:
Fatemeh Yarahmadi *1, Pegah Yaali2
1Department of Plant Protection, Faculty of Agriculture, Khuzestan Agricultural Science and Natural Resources University, Mollasani, Ahvaz, Iran
2Department of Plant Protection, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran
Abstract:
The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), is one of the most important pests of stored grain products in the tropics. Chemical control using synthetic insecticides and fumigants is the primary method for managing the pest which has resulted in serious adverse effects on mammals' health, other non-target organisms and environment. Efforts have been focused on botanical products essential oils (EOs) as possible alternative to conventional insecticides. Some limitation including low physical stability, poor water solubility, and quick degradation in the environment have made EOs application impractical. Nanotechnology method including nanoformulations is a unique strategy to overcome the mentioned limitations. In this study, contact toxicities of pure Eucalyptus globulus Labill essential oil compared with two nanoemulsions of E. globulus (Span 80® and gum Arabic) were investigated on T. castaneum adults. The ultrasonic emulsification method was used to prepare nanoemulsion Span® 80 and gum arabic. Results indicated that toxicity of pure essential oil (LC50=6.82ppm) was significantly higher than two nanoemultions, without any significant differences. After 48 h exposure period, the LC50 values of Span® 80, gum arabic nanoemulsion and pure EO were 15.05, 5.55 and 5.02 ppm, respectively. Also, after 72 h exposure period, the Span® 80 nanoemulsion had the highest values 4.96ppm, whereas the pure EO and gum arabic nanoemulsion values were 6.17 and 11.04 ppm, respectively. Evantually, our findings indicated that Span® 80 nanoemulsion improved the stability of E. globulus essential oil and increased its efficacy against T. castaneum. Our finding showed that the nanoemulusation (Span® 80) can be applied, as contact insecticide, in the silage regarding to its less effect on stored product qualities including their test, smell and etc.
Keywords:
red flour beetle, grain storage products, slow-release formulation
Status : Paper Accepted (Poster Presentation)